What is the best method to preserve the individuals of Daphnia magna prior to DNA extraction?

More Nikola Petkovic's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

What is the Standard Procedure for measuring Total Lipid concentration in Daphnia magna?

currently working on a project involving Daphnia magna and I am intertied in measuring their total lipid concertation. I was hoping to get some insights or advice on the standard procurers for...

23 May 2024 8,846 1 View

Why we do not feed daphnids during 48-hour acute toxicity assay?

Why we do not feed Daphnia during the 48 hours. Will that not affect them?

01 April 2024 685 0 View

What are the methods or chemicals we can use to inhibit cell proliferation of Bacillus sp.?

Hello scientists, microbiologists, and respected researchers, I am looking for methods or chemicals that can use to inhibit the cell proliferation of Bacillus sp. in systems containing green...

14 August 2023 7,631 0 View

Hello Everyone, please what is the best method for extracting DNA from the gut of Daphnia magna?

I presently use the powersoil pro kit for the extraction but I am not getting my desired result maybe cos the starting material (Daphnia magna gut) is too small. i'd like to know what methods has...

27 June 2023 1,021 1 View

Sources for Daphnia Magna in the US?

Looking into the possibility of doing new ecotoxicology studies with Daphnia Magna alongside my fish work. I have seen a few sources that seem to mostly market toward teaching and classroom labs....

30 November 2022 962 4 View

Is Dapnia_magma classified as a bacterium? What went wrong with the taxonomy of 16S rDNA gene amplicon sequencing?

We recently used QIIME2 to perform bioinformatics analysis on the results of PCR amplification and sequencing of 16S rDNA, and I found Daphnia_magna as one of the species assigned as shown below:...

06 May 2022 4,765 2 View

Is there any traditional method to isolate genomic DNA for Zooplankton other than the kits ?

Zooplankton are like Moina, Daphnia etc

30 July 2021 3,568 0 View

Can anyone recommend me the best medium for cultivation of Chlorella vulgaris?

I intend to grow C. vulgaris as a food source of Daphnia magna.

27 May 2021 2,658 3 View

Can I feed my daphnia culture with dry spirulina powder (available in drugstores) and yeast?

I am culturing daphnia which will be used for toxicity studies. According to several protocols, they should be fed with live Chlorella. I am looking for an easy feeding method, and found that in...

03 May 2021 1,710 3 View

How to express the measurement uncertainty with the confidence interval (Daphnia magna ISO6341)?

Hello, I am accrediting the acute toxicity assay with Daphnia magna ISO 6341; may you suggest how to express the measurement uncertainty with the confidence interval? Thanks in advance.

09 March 2021 5,461 2 View

Judson Rosa

Alcohol concentration above 92.8° INPM, (96°GL) and just in case, put it in the refrigerator. This is the preservation method I used for DNA from Marine Copepoda.

A. Ye. Kaglyan

Excuse me, I am studying radionuclide contamination of fish in the Chornobyl Exclusion Zone. Unfortunately, I will not be able to help you. Excuse me.