How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

Analysis reproduction data in chronic toxicity tests with Ceriodaphnia dubia?

Hi everybody. I am facing an issue about reproduction data analysis in chronic toxicity test with C.dubia. US EPA Method 1002.0 (...

13 March 2020 1,932 4 View

Which light intensity is optimal for cultivation of Daphnia magna?

The animals I intend to use in the experiments will be cultivated in environmental chambers under various temperature regimes and illuminated with cool-white LED bulbs (which intensity will be...

19 November 2019 1,894 2 View

Calculating Growth and Ingestion Rates?

I am looking for an easy-to-understand growth and ingestion rate paper or excel file for beginner ecology students. Everything I am finding is a bit too advanced for them. Does anyone have a nice...

01 February 2019 5,700 1 View

Which statistical analysis could I use to show that my data is statistically significant?

I am trying to determine which statistical analysis I could use to show that after I add certain drugs, that the heart rate of Daphnia changes significantly. I do not know what test to use because...

31 December 2018 3,755 9 View

Has anyone experience with dying Daphnia culture in an incubator with artificial light?

We have a problem with Daphnia cultures. If they are on natural light (in the room behind the windows), there is no problem. To the contrary, daphnids die under artificial light inside an...

31 December 2018 8,988 3 View

Is Daphnia Dried Powder Used in Fish Meal? And is it worthwhile?

I want to know, is it possible and economical to use Daphnia dried powder in the fish diet?

11 December 2018 9,957 3 View

Daphnia in European cities?

I am looking for Daphnia in European city centers and would like to reach out to all you zooplankton lovers who might have encountered Daphnia magna in urban ponds anywhere between Sweden and the...

28 November 2018 1,309 15 View

Daphnia in European cities?

I am looking for Daphnia in European city centers and would like to reach out to all you zooplankton lovers who might have encountered Daphnia magna in urban ponds anywhere between Sweden and the...

10 November 2018 7,013 13 View

Is there anyone who can give me any information about this parasitic fungus on Daphnia?

I found this infected individuum during routine processing samples from fishponds. I saw only one infected specimen in the sample. It was first time when I recognized something like that on...

04 May 2018 2,013 10 View