How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What is the chemical composition of chlorellin?

The chlorellin is the chemical compound which is first extracted from Chlorella vulgaris by Pratt in 1944. Spoehr in 1949 wrote that it is a mixture between fatty-acids and hyrdocarbons and its...

25 January 2021 8,538 1 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

Alternative to use of ethanol in extraction process?

Dear all., I am using ethanol as part of the extraction process of Chlorella vulgaris carbohydrates. Since I am trying to scale up a bit the whole process, I should use much more ethanol, that in...

27 August 2020 1,875 5 View

Can Tomato yellow leaf curl virus infect brassicas and beets?

It is stated in https://www2.ipm.ucanr.edu/agriculture/tomato/tomato-yellow-leaf-curl/ that TYLCV can infect a wide range of plant species, including peppers (Capsicum annuum), eggplants,...

20 May 2020 1,761 3 View

Analysis reproduction data in chronic toxicity tests with Ceriodaphnia dubia?

Hi everybody. I am facing an issue about reproduction data analysis in chronic toxicity test with C.dubia. US EPA Method 1002.0 (...

13 March 2020 1,932 4 View

How to identify pure green microalgae biofilm on SEM image?

I have SEM image of green microalgae Chlorella sp but I am not sure whether the green microalgae is pure or contaminated with other microbes.

18 February 2020 1,476 4 View

Which light intensity is optimal for cultivation of Daphnia magna?

The animals I intend to use in the experiments will be cultivated in environmental chambers under various temperature regimes and illuminated with cool-white LED bulbs (which intensity will be...

19 November 2019 1,894 2 View

How can i extract protein from microalgae (particularly spirulina and chlorella)?

Hello all, I need a facile method to extract protein from microalgae with a high yield. I tried HCl and NaOH solution along with sonication . But the yield was low. Would you have any suggestions...

06 July 2019 5,880 5 View

Hello there. Has anybody had any experience with the accelerated ageing test on Beta vulgaris seed? Is prewashing necessary?

Beta vulgaris has an inhibitor in it's outer woody seed coat that is best prewashed out before commencing a germination test. Just wondering if you need to do that prewash step if you are going to...

04 May 2019 9,409 4 View