Hello Everyone, please what is the best method for extracting DNA from the gut of Daphnia magna?

28 June 2023 1 1K Report

I presently use the powersoil pro kit for the extraction but I am not getting my desired result maybe cos the starting material (Daphnia magna gut) is too small. i'd like to know what methods has been used that yields a good result.

Nadia Côté

I use the MasterPure Complete DNA and RNA purification kit from Biosearch technology. To carry out my DNA extraction, I use 10 adult daphnia which I crush with our small homemade device which vibrates in the lysis buffer of the kit. I advise you to read the following paper, we based ourselves on it. Optimization of DNA extraction from the crustacean Daphnia by Athanasio published in 2016 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867708/)

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