I want to get it on on MEA, PDA or any other media
or are there any methods without using media?
Dear Ismael, yes it is about fungi. I am working on order Pleosporales.
Pleuosporales such as Pleuospora are plant pathogens. Try to grow them on sterilized plant material such as potato, carrot, tomato and others, in tubes with adequate moisture, and incubate at 22 degree C.. Observe periodically for sporulation under microscope. You may contact Commonwealth Mycological Institute, Kew. They do lot of work.
Dear colleague,
You should take into account that fungi can be either homothallic or heterothallic. The former can produce a sexual stage without crossing compatible isolates, but the latter need crossing experiments.
In Pleosporales both kinds of species exist. To obtain the sexual stage it is advisable to use poor media, as Prof. Thammaya pointed out. Oatmeal agar, potato-carrot agar, or even water agar with sterilized plant material (ideally the plant that the fungus is associated to).
You can inoculate plates with single isolates, as well as different combinations of pairs of isolates of the same species, separated by a few cm in each plate. Temperatures around 20ºC are usually OK, but it depends on the genus/species. Some genera require to be stimulated by near UV light to produce ascomata, but many do produce them in the dark or with diffuse daylight.
Good luck with your research!
Hugo
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