I am trying to isolate the SlpA (Surface Layer Protein A) protein from Lactobacillus brevis with the Laemmli cell lysis buffer. I am still unable to get the precise protein band in SDS PAGE run at the 44.31 KDa. Please suggest any specific method to isolate the cell surface proteins. And also suggest the protein storage method for a long time.
Try brief (15 to 30 seconds) treatment with 15% ethanol at 25 and 37 C. Such treatment will help releasing loosely bound proteins from membrane surface (see attachment). Also try treatment with various concentrations of NaCl (from 0.1 M to 1M) under cold condition with varying time to find the condition suited for your purpose.
Thank you very much Dr Tushar Kanti Ray for the nice answer, the protocol is working well.
- Badges
- Science method