What is the best method to identify unknown proteins, after running through SDS PAGE?

Protein-aptamer gel electrophoresis help, please??

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What are some important techniques for protein induction and SDS-PAGE analysis?

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SDS page software for analysis?

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How to compare different concentrations of nanoparticles for characterization from ICP-MS?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

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Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

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Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

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How can I represent SDS-PAGE results for machine learning analysis?

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I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

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Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

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Adam B Shapiro

Article ‘Routine Identification of Proteins from Sodium Dodecyl Sulf...

Sushant Kumar Sinha

Yes, you can perform Pepsin/Trypsin digestion and then MALDI-TOF-MS to identify your protein of interest. You can follow the detailed protocol from the attached file.

Manavi Ekanayake

Thank you for all experts, Adam B Shapiro , Sebastian Schmitt

and Sushant Kumar Sinha for your valuable suggestions. This will help me lot.

Farhana Rumzum Bhuiyan

Dear Manavi,

You can collect the bands from gel through excision and purify the protein bands. Then digest your protein into peptides using standard methods. Finally, you can choose LC-MS/MS method to know the unknown proteins.

Good Luck