What is the best method to extract DNA from microbiota in murin models?

More Baptiste Lamarthée's questions See All

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

What is the spatial resolution of paleomagnetic data for paleogeographic reconstructions?

Are there any references stating clearly the limitations (especially lower limit) of the spatial resolution of paleomagnetic data when used for paleogeographic reconstructions? i.e., how many...

16 April 2024 8,051 0 View

How would (a) hypothetic (geological) structure(s) of a specific age look like at present after having been deformed by known tectonic events?

Is there a term or expression in the geosciences (or any other field?) that means "to imagine how (a) hypothetic (geological) structure(s) of a specific age would look like at present after having...

18 February 2024 9,162 5 View

Any paper/collaborator with info on the stratigraphy west/southwest of the Faroe Islands?

Hi, we are looking for potential tectonic structures around the Faroe Islands on seismic data and are looking for papers detailing the stratigraphy there (especially west and southwest of the...

21 December 2023 4,435 1 View

What are those (THP-1 contmination with moving cells) ?

Dear all, I am cultivating cells in RPMI-1460-FBS 10%-P/S 1% and I infected them with PMA 100 ng/ml during 24 hour and then maintained them with the regular medium, this is what I got after 4...

16 September 2023 6,464 0 View

How to generate single corrosion pits on a steel plate?

In order to assess the effect of ocrrosion pits on fatigue of a steel material, I would like to know if it is possible to create a single corrosion pit on a sample

05 September 2023 9,889 3 View

Fracture mechanics - C(T)XX test specimen?

What do the two digits following C(T) in C(T) specimen nomenclature? For instance, does C(T)50 refers to a C(T) specimen with W = 50 or B = 50 ?

02 August 2023 8,969 0 View

I would like to implement AI on DEM analysis but i have no clue to where to start. Any help?

I have 24 square kilometer DEM with known structures and still have a topographic survey (satellite derived contours) of around 1 sq kilometer of unknown territory (forested land). How could I...

04 May 2023 8,250 4 View

In facture mechanics, why is it necessary to do a precrack in the test specimen? Why the notch is not enough?

In fracture mechanics, there are several test specimen, such as the C(T) specimen, and when assessing the crack growth behoavior, to determine the threshold stress intensity factor, a precrack is...

31 January 2023 9,397 1 View

What is the threshold stress intensity (Kth) factor for nitrided and carburized steels?

What is the minimal acceptable defect size before crack propagation in nitrided and carburized steels?

06 December 2022 7,160 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Mohsen Sohrabi

it may help you

The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0088982

Baptiste Lamarthée

Thank you very much!

Finally, I used the QIAGEN kit "DNA Fast stool extraction" and it worked properly for my experiments.

Alexander Sasha Vlassov

PureLink Microbiome DNA purification kit (Invitrogen) works really well on stool samples, and has protocols for other samples as well (saliva, urine, swabs)