What is the best method to extract DNA from microbiota in murin models?

More Baptiste Lamarthée's questions See All

Can an editor reject or accept a paper based on the poor quality of the cover letter?

1. What is the importance of cover letter when submitting an article to a journal? 2. Please enumerate the major points which a good cover letter should include. 3. Can an editor reject or...

13 July 2020 5,691 15 View

Where can I purchase microalgae cultivation bags ?

Do you know any suppliers or companies which are selling plastic bags for algal cultivation ? These bags have a volume capacity ranging from 1 to 50 L and you can even install an aeration system...

19 November 2019 2,250 3 View

How can I internalize proteins (NO DNA) into living bacteria (E. Coli) ?

Hello, I would like to study the effect of adding a certain protein to living E. coli to study it's effect, not by producing the protein, but and I have to deliver it by internalization. I DON'T...

23 April 2017 1,428 1 View

How to plot XY history output in a cylindrical CSYS (Abaqus) ?

Hi guys ! I'm looking for a way to plot XY history output results in a cylindrical CSYS (it's ok with field output but not for history..). I'm working on a pipe geometry where forces are applied...

16 March 2017 5,563 4 View

In Abaqus Standard, is it possibile to automatically get the "pressure" field [(Sigma.n).n] at the predifined surface of a tie constraint ?

I want to know if at a non-planar surface tie constraint, I have traction or compression between my two 3D parts, I need to get : (Sigma.n).n Sigma being the stress tensor and n being the normal...

01 March 2016 3,898 3 View

No title

Where ^N1(x)=Sum(1,xj=1), j=1,N and xj=1 for j in N1, 0 otherwise

20 May 2014 5,566 1 View

Renaming BioInformatics?

Following the "successful" debate on the definition of bioinformatics and its elusive scope. It might be time to look for a more appropriate name to cover the many forms it takes. So far, none of...

18 August 2013 2,184 10 View

Define Bioinformatics

As Genetics has been ever expanding, the term bioinformatics has become over inclusive. It can go from Statistical Genetics to Computational Biology, far away from data mining and algorithmic....

11 February 2013 9,529 19 View

Grey ware ceramic

Hello, In the framework of a broader article on Hellenistic ceramics in Central Asia, I worked on grey-black paste ceramics in order to try to establish connections between Central Asia and the...

01 January 1970 2,864 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Mohsen Sohrabi

it may help you

The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0088982

Baptiste Lamarthée

Thank you very much!

Finally, I used the QIAGEN kit "DNA Fast stool extraction" and it worked properly for my experiments.

Alexander Sasha Vlassov

PureLink Microbiome DNA purification kit (Invitrogen) works really well on stool samples, and has protocols for other samples as well (saliva, urine, swabs)