What is the best method to evaluate the effect of an antiseptic on laboratory animals (mice or rabbits) without use of experimental wounding ?

10 November 2018 0 10K Report

The objective is to have results without invasive surgery or tissue damage

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MANOVA for IV and DV catergorical data?

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For ns pulsed high voltage power supply (for atmospheric and vaccum plasma), which is better to order: positive or negative pulse? and why??

Many thanks in advance.

15 January 2024 6,145 4 View

What practical methods are used for abdominal ultrasound in ruminants?

We're talking about repeatable teaching methods and clinical practice methods.

07 June 2023 6,404 1 View

Please remove my article Methylene Blue Removal Using Black Cumin Seeds Waste: Experimental and Theoretical Investigations from the SSRN Electronic ?

Hello, I am hereby informing you that the article entitled: Methylene Blue Removal Using Black Cumin Seeds Waste: Experimental and Theoretical Investigations, has been hacked by other people . I...

30 March 2023 1,053 2 View

Difference hardness and elastic modulus?

hallo every one, I know that the hardness of a substance means how strongly it can resist against penetration of a harder substance and the E means after the release of a stress the material will...

21 February 2023 5,182 3 View

Adhesion Theory that describe the adhesion of the photo resist on wafer?

Hello every body, can some one please tell me which adhesion theory could describe the adhesion of photo resist on Wafer?? and with HMDS? thank you very much!!

10 January 2023 722 0 View

Effect of the implantation on hardness from the photo resist?

Hallo, i did a nanoindentation on an implanted CAR and i found that when the CAR has a thicker layer, then it is less hard. Does anyone know why?? thank you very much!!! best regards chiko

28 August 2022 4,657 0 View

What can affect the hardness of CAR (layer thickness)?

Hello, i did a nanoidentation experiment : 1 CAR with 3 different layer thicknesses. My results show that the CAR is harder when it has thicker layer.. I can't find the reason in the...

11 August 2022 2,888 0 View

What can affect the adhesion of CAR?

Hello, i am making researches about what can affect (or what could improve) the adhesion of CAR if any one have articles for this topic, please let me know. Thank you very much!! best regards chiko

03 August 2022 7,146 3 View

There is a database/resource to search whether the plant species are polyploid or diploid?

There is a database/resource to search whether the plant species are polyploid or diploid. I have many plant species and want to know which one is polyploidy/diploid.

23 June 2022 4,890 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Cell culture model of Acute kidney injury?

Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...

29 July 2024 5,622 3 View