The objective is to have results without invasive surgery or tissue damage
Hi all, I am trying to test my polymer stability in biological medium. I know how to make 1% /5% etc of BSA in buffer but I am not sure how do we actually decide this percentage volume. Is there...
05 January 2021 649 2 View
Hi all, I am trying to test stability of my electrode immersed in solution. I intent to keep them in oven for 3 weeks as to represent accelerate ageing. However, my worry is that the solution...
28 October 2020 8,351 2 View
Hello, I synthesized chitosan nanoparticles loaded with a plant extract. For confirming presence of the extract in the nanoparticles I did a FTIR analysis. Now my question is: What is...
24 January 2020 4,402 2 View
I am looking to start working with the isogeometric method to analyze mechanical behavior such as beam, plate, shell and other structures of complex shapes. can you help me by giving me simple...
28 December 2019 2,729 5 View
Dear all, I have ordered a peptide (with 32 aa) for chemical synthesis, but I dont know after receive a peptide how to ensure from its sequence. Which method(s) are available for my purpose?...
09 December 2019 3,784 3 View
Hi, I am currently pursuing my Phd study whereby my topic related to fintech and the effect towards green banking, and I am now in the process of understanding the LR about Fintech. Since Fintech...
08 December 2019 1,391 5 View
I found many plagiarism programs that allow only 150 words for checking. I search for a free download plagiarism checker program (not trial). Thanks
20 March 2019 5,708 6 View
I want to start working on the generalized differential quadrature (GDQ) method to solve the problem of the mechanical behavior of the beams, the plates and the shells. Dear colleagues , I...
13 November 2018 7,650 3 View
Hi every one, I would like to synthesis of Cu Nanoparticles using plant extract, but in previous works different precursors have been used ( chloride and sulfate salts) , based on your experience...
17 October 2018 5,926 3 View
Hi, I have a text that include some number in latest line and I would like to extract this numbers to a separate file. Can anyone help me in this regard. A sample file of mu results is attached....
29 May 2018 3,620 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View