I am particularly interested in the fixation and H&E processing steps that give the best chance of detecting edema in rodent organ (heart, lungs, liver, pancreas, kidneys, intestine) tissue. Can anyone help?
Hans-Joachim Franz Schurek
Edema is a functional condition and it is not easy, to capture this condition in the histological preparation. The question is how to freeze this functional state; certainly not with the usual histological techniques. In the case of the kidneys, normothermic perfusion fixation with glutaraldehyde, a colloid-osmotically adequate additive such as hydroxyethyl starch, is recommended.
HJ Schurek, W Kriz: Morphologic and functional evidence for oxygen deficiency in the isolated perfused rat kidney. Laboratory Investigation 53: 145-155, 1985
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