How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Is there no general protocol for vaccination study?

Hi, I searched vaccination study to know which MLD50 is suitable for influenza virus study in pubmed. But why every vaccination and challenge conditions are different? Is there no guideline or...

16 January 2021 7,984 3 View

How can I generated Simulated/Predicted Effects from an MRQAP model?

I want to generate some nice prediction plots from my MRQAP model. I've laid out my process below, and would be very grateful to get anyone's insight, as I'm not seeing much written about this...

05 November 2020 1,215 2 View

Best Flow Cytometry Rat Spleen processing for Macrophages?

I'm trying to design a protocol to process rat spleens to maximize macrophage yield for flow cytometry. Currently I plunge my spleens through a filter and incubate with lysis buffer before...

29 October 2020 5,671 2 View

Does anyone know of any sets of surface colour spectral reflectance curves in the visible light region (i.e. from 400nm to 700nm) available online?

I think there are physical limitations on the spectral reflectance curve that can be achieved by a physical object. The small number spectral reflectance curves I have found online seem to share...

10 September 2020 5,357 5 View

Is it OK culture E.coli at RT for overnight?

For plasmid DNA isolation, E.coli was incubated last night. But incubator doesn't work and I just knew it this morning. The incubator fixed now. Is it okay for my experiment?

28 April 2020 2,931 10 View

Is there a way to batch-convert mass spectrometry cdf files to mzML files?

I have a set of roughly one hundred cdf files from a mass spectrometry experiment. I would like to analyse them using MetaboAnalystR, which requires mzML files as input. I know I can convert each...

28 March 2020 1,794 6 View

Is it possible to predict the intermetallic compounds/phases that will be formed in an alloy (i.e. maraging stee) only from its chem. composition?

I am trying to determine an intermediate phase/compounds that will be formed and its fraction (if possible) as well as their sequence (If more than 1 phase) from its chemical composition...

24 March 2020 1,889 4 View

Is there a free Raman Spectra database that I can search for a set of given peaks?

Is there a free Raman Spectra database that I can search for a set of given peaks? Thanks in advance for pointing the way.

22 December 2019 4,237 4 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View