Timothy Dreyer

3 Questions 6 Answers 0 Followers

Questions related from Timothy Dreyer

What is the best method to deliver a 20kb transgene to mammalian cells for protein expression?

I'm aiming to express a protein in mammalian cells via a transgene. I've looked at Herpes Simplex Virus (can insert large sequences into viral genome) and hydrodynamic transfection for delivery of...

31 January 2020 3,207 0 View

Would there be any difference between using human Fc fusion proteins vs mouse Fc fusion proteins in a mouse model?

Multiple research groups have tested petide-human Fc fusion proteins in mouse models, with no apparent adverse effects. However, I can't find any information on the difference between using human...

09 February 2019 8,144 1 View

How to positively select HEK293T suspension cells transfected with pcDNA3.1+ plasmid?

I want to create a stable suspension cell line that expresses my protein. I am aware that both HEK293T cells and pcDNA3.1+ are resistant to G418. My budget does not allow for custom plasmid...

03 November 2015 2,000 13 View