Hi Amany Gamal . To select the most accurate and sensitive viability assay for your treatment, it's important to consider how your compound affects the cells as different assays measure different aspects of viability.

If your treatment is expected to affect metabolic activity, MTT or Alamar Blue would be appropriate. If you're assessing membrane integrity, Trypan Blue exclusion or LDH release assays are suitable. For detailed apoptosis studies, you can consider Annexin V/PI. In many cases, combining multiple assays can provide a more comprehensive understanding of the treatment's effects.

Hope this helps!

I would suggest MTT