Dear Alok

Ultracentrifugation is the most accepted method.  Please visit

http://www.ncbi.nlm.nih.gov/books/NBK19361/

Best wishes

Ravi

I'm not sure of the best method. I have used PEG based concentration methods and worked fine for me. 

For storage follow the instructions in paper (link given below)

http://www.ncbi.nlm.nih.gov/pubmed/19300443

In my experience, resuspending virus in serum reduces its infectivity. So i suggest resuspend virus in either incomplete RPMI or DMEM or cell culture media without serum. You can also resuspend in PBS or tris buffer. Then snap freeze and store at -80 degree Celsius.

Hope this helps

I`d say, ultracentrifugation. Cutoff coliumns were no good in my hands - you co-purify a lot of serum proteins from foetal bovine serum added in meduim - thus making the preparationin your Ultracon viscous as sticky rubber.

hi,

it is possible both to use a precipitation approach such as the new

Intact Virus Precipitation Reagent or a Dynabeads approach taking advantage of the charge of the virus - Dynabeads™ Intact Virus Enrichment (also suitable for automating the virus enrichment in a very quick way - 20 min)

kind regards

ketil