Bivek Timalsina

10 Questions 46 Answers 0 Followers

Questions related from Bivek Timalsina

What is the best method of determination of possible cellular targets of therapeutic peptides on cancer cells?

There is the specific interaction between target cells and therapeutic agents. I am focussing on the determination of possible cellular targets of therapeutic peptides. I think MS and RNAseq...

07 July 2015 5,246 2 View

How can we remove bacterial contamination from a contaminated HEK cell line growing in a T-25 flask?

I am growing a HEK-293 cell line using DMEM medium + 10%FBS + 1% PS(Penicillin and Streptomycin) antibiotics in a T-25 flask. I encountered bacterial contamination in cell lines which made the...

02 February 2014 9,410 80 View

What are the transfection conditions for pIndie-C1 DNA (related to HIV type 1 subtype C clone) to HEK-293 cells using Lipofectamine?

I did transfection to 90% confluent HEK-292 cell line with pNL4-3 DNA and p-Indie-C1 DNA using lipofectamine 2000 in 6 well culture plate. My experiment recipe is as: 1. 5 ul Lipo +250 uL DMEM...

01 January 2014 9,831 17 View

Which competent cell is suitable for transformation of pIndie-C1 DNA and what are the transformation criteria?

I have done transformation of pIndie-C1 DNA (using 100 uL ; 0.6 ug/uL) in Top 10 competent cells using heat shock method. 400 uL was transferred to 250 uL LB broth and 100 uL was plated in LB...

01 January 2014 4,254 3 View

How we can correlate Stress granules and P- bodies?

The nontranslating RNA molecules leads to the formation of granular structures like Stress granules and P-bodies. They are specialized strucutures. How these are interrelated with each other. In...

10 October 2013 7,469 3 View

Why does RNA Polymerases of (-) strand viruses copy viral RNAs only when they are present in the nucleocapsid?

VSV genomic RNA is a template for RNA polymerase only when it is bound to the nucleocapsid protein N.

09 September 2013 1,148 1 View

What will happen to Huh-7 cell line if we use 5% CO2 for DMEM medium rather than 10% CO2?

We have to optimize the growth characteristic for cell line culture. Ph is a very important factor for this. We want to use both RPMI and DMEM medium in the same CO2 incubator. But the CO2...

08 August 2013 5,925 5 View

Can all + ss RNA genome can act mRNA & directly undergo translation?

RNA viruses are classified according to the sense or polarity of their RNA into negative-sense and positive-sense, or ambisense RNA viruses. Positive-sense viral RNA is similar to mRNA and thus...

08 August 2013 7,485 10 View

Why Chimpanzees are not infected with Malaria Parasite, Plasmodium falciparum though Human and Chimpanzee are more closely related?

It is found that, when the blood from Human infected with Plasmodium falciparum is injected into the Chimpanzee, they don't show any severe effect as in Human and similarly when the blood from...

04 April 2013 7,028 6 View

How is scoring done in BLOSUM 62 and PAM 120 substitution matrices?

Substitution matrices for aligning Protein sequences.

04 April 2013 3,527 3 View