best is size exclusion chromatography. If not accessible, dialysis should also work (1000 cut-off at least)

best is size exclusion chromatography. If not accessible, dialysis should also work (1000 cut-off at least)

Dear Mayank

You can use Titanium Dioxide.

Very recently, a titanium dioxide (TiO2) microcolumn has been developed as an efficient method to enrich phosphorylated peptides.

Please use the following article with this title:

"Removal of Polyethylene Glycols from Protein Samples using Titanium Dioxide"

Cheers

What is your biological sample? If a cell or tissue, just centrifuge and wash off with buffer or water. If a protein then use chromatography methods to purify. If DNA you could just use a PCR clean up kit or Ethanol precipitation.

Sample is proteins..

Dear Mayank

PEGs are prepared by polymerization of ethylene oxide and are commercially available over a wide range of molecular weights from 300 g/mol to 10,000,000 g/mol.

What is the Mw range of your proteins and what is the Mw of used PEG?

By these information, you can also provide more helps and discussions.

my sample is small peptides and PEG is 400...

What about ether precipitation of peptides...?

You can use CHROMATOGRAPHY to remove PEG from your samples and purify the peptides completely.

Please find the attached file.

Crystallize your macromolecule and remove crystals from solution