Mayank Saraswat

11 Questions 43 Answers 0 Followers

Questions related from Mayank Saraswat

Is it possible to make a phage display library in which every gene is fused to a unique bar code?

If you make a phage library, is it possible to introduce a unique peptide bar code in each phage?

10 October 2013 7,741 14 View

Is it possible to selectively modify hydroxyl groups of serine, threonine and tyrosine?

Is there are way to modify serine, threonine and tyrosine hydroxyl groups?

10 October 2013 5,084 13 View

How to avoid contamination of non-glycosylated proteins in lectin affinity chromatography?

My sample has some sticky proteins and they are also getting enriched when I am enriching glycosylated peptides using lectin affinity chromatography. How to avoid this contamination?

10 October 2013 6,848 13 View

How can one block only specifically the carboxylic acid groups in proteins?

wWhich reagent can be used to rapidly and easily block free COOH groups in proteins?

09 September 2013 248 14 View

Can exosomes be membrane disrupted using sonication?

Can exosomes can be disrupted by sonication using a sonicator which is fixed strength water bath based? If so then for how long do you need to sonicate?

09 September 2013 4,265 3 View

What is the best method for PEG removal from biolgoical samples?

How can PEG400 be removed from biological smaples?

09 September 2013 1,428 10 View

Are all IgG either basic or acidic proteins...?

Does the change in variable region changes PI?

09 September 2013 6,083 1 View

Can you get rid of HCl by speed vacuum drying?

Is it possible to remove HCl from protein samples using speed vacuum drying?

09 September 2013 8,430 5 View

How much normality of HCl can peptides tolerate upon heating before they hydrolyse?

Is it possible to heat proteins in 100 mM HCl without hydrolysing them? Usually the reaction is done at 110 C at 6M HCl.

09 September 2013 4,767 1 View

Could someone please explain the protocol to capture cysteine containing peptides on thiol agarose?

How long should the incubation be, what buffer could be used, etc.?

09 September 2013 8,215 3 View

Can very small peptides (Trypsinized) be precipitated using cold acetone, or is it good for only bigger proteins/peptides?

Is it possible to precipitate trypsin generated fragments of proteins? By the standard protocols or are there any modifications? Is it a problem if sample has .1% formic acid?

08 August 2013 1,019 10 View