Could someone please explain the protocol to capture cysteine containing peptides on thiol agarose?

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Steingrimur Stefansson

Here is information on using Thiol Sepharose.

https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314787424814/litdoc71710600AF_Web_20110831133429.pdf

Vojtech Tambor

Hi! I worked on this for a while and optimized the protocol using radioactive cysteine peptides, you can find the paper attached. Cheers, Vojtech

Mayank Saraswat

Thanks a lot vojtech,,, :)