What is the best method for PEG removal from biolgoical samples?

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Nicole Nischan

best is size exclusion chromatography. If not accessible, dialysis should also work (1000 cut-off at least)

David Bateman

What is your biological sample? If a cell or tissue, just centrifuge and wash off with buffer or water. If a protein then use chromatography methods to purify. If DNA you could just use a PCR clean up kit or Ethanol precipitation.

Mayank Saraswat

Sample is proteins..

my sample is small peptides and PEG is 400...

What about ether precipitation of peptides...?

Françoise Bonneté

Crystallize your macromolecule and remove crystals from solution