Non-specific labelling with Click chemistry reaction?

I am working on identification of a small peptide in brain tissue. We have modified the peptide with alkyne modifications for azide-based clicking. For Click reaction we have used 50 uM CuSO4,...

23 September 2019 8,052 3 View

Problem in protein purification?

I have a doudt in adding protease inhibitor. Should i add the cocktail both in cell lysis buffer and column buffer to prevent my protein from degradation during purification and while storage? or...

06 September 2016 4,086 4 View

Can a single amino acid codon changing of a recombinant protein effect protein expression in E.coli?

i have change an amino acid codon asparagine to aspartic acid in my recombinant protein plasmid then transformed in bl21(de3) and used it for expression of my new mutant protein.I expressed it...

19 August 2016 8,032 6 View

What is the minimium possible time to reach OD 0.5 at 600nm by an E.Coli ?

08 August 2016 6,627 10 View

Is there any way I can improve AHL stock preparation for quorum quenching OPA based enzyme assay?

25 January 2016 2,213 3 View

Can someone suggest another scholar besides Hall, that defines hegemony as an ideological force that can produce "the othering"?

In this I mean other profound scholars that have defined hegemony as having the ability to represent those being dominated in a particular regime of representation.

28 January 2015 9,543 6 View

I am looking for scholarly literature on war journalists from developing countries. Can someone help and recommend some work ?

My readings for the past one year has revealed that war journalism is a predominantly western based. But have the developing world indulged in studying their journalists' who cover wars

06 January 2015 9,996 14 View

Can someone help me grasp the concept of memory in journalism?

Beyond Zelizer's work

05 January 2015 1,083 3 View

What is the colour of E coli colonies on EMB agar?

We got purple black, pink and white colonies on EMB agar plate. From that how we will detect E.coli.

10 July 2013 133 41 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View