Pushpa Kakchingtabam

9 Questions 5 Answers 0 Followers

Questions related from Pushpa Kakchingtabam

How to separate his-tag from fusion protein clone in pRSET A vector?

I have pRSET_A vector fused with human Prdx6. My vector has enterokinase cleavage site inorder to separate his-tag. By using enterokinase ,i have plan to remove His-tag from my protein.Now i can't...

11 April 2018 162 3 View

Has anyone done NADPH dependent peroxiredoxin (prdx6) assay?

i have purified prdx6 protein and now working on its activity assay using nadph, glutathione and glutathione reductase. its substrate is hydrogen peroxide. im not getting the actual result. Is...

19 May 2017 3,373 1 View

What could be the best storage temperature for stock NADPH?

I m using NADPH for assay of peroxiredoxine. I m confuse in storage temperature of stock solution of NADPH. 4 degree or 20 degree be the best. ? and at what solvent will it best be stable for...

04 May 2017 3,676 3 View

What are the possible troubleshooting faces while doing peroxidase activity assay?

I'm planning to do NADPH dependent method peroxiredoxin activity assay. Can anyone elaborate on Do's and Dont while doing such an activity assay? thank you

18 January 2017 9,324 3 View

What is the best method for assaying the activity of peroxiredoxine ?

I have my purified protein Peroxiredoxin 6,which is a peroxidase enzyme. Now I'm confused with method use for checking its enzyme activity. Can anyone suggest me a better method?

27 October 2016 675 6 View

Problem in protein purification?

I have a doudt in adding protease inhibitor. Should i add the cocktail both in cell lysis buffer and column buffer to prevent my protein from degradation during purification and while storage? or...

07 September 2016 4,143 4 View

Can a single amino acid codon changing of a recombinant protein effect protein expression in E.coli?

i have change an amino acid codon asparagine to aspartic acid in my recombinant protein plasmid then transformed in bl21(de3) and used it for expression of my new mutant protein.I expressed it...

20 August 2016 8,064 6 View

What is the minimium possible time to reach OD 0.5 at 600nm by an E.Coli ?

Im using terrific broth(TB) to grow e.coli for recombinant protein expression.it take minimium of 3 hrs to reach OD=0.5 at 600nm. what might be the possible problem behind this?

09 August 2016 6,667 10 View

Can growth of bateria be slow if the media is not a good quality?

I'm working on protein induction in e.coli using IPTG and for me the protein is not properly induced. I think E.coli is not having good growth. After doing overnight induction, the growth media is...

15 July 2016 6,291 2 View