Non-specific labelling with Click chemistry reaction?

I am working on identification of a small peptide in brain tissue. We have modified the peptide with alkyne modifications for azide-based clicking. For Click reaction we have used 50 uM CuSO4,...

23 September 2019 8,052 3 View

Problem in protein purification?

I have a doudt in adding protease inhibitor. Should i add the cocktail both in cell lysis buffer and column buffer to prevent my protein from degradation during purification and while storage? or...

06 September 2016 4,086 4 View

Can a single amino acid codon changing of a recombinant protein effect protein expression in E.coli?

i have change an amino acid codon asparagine to aspartic acid in my recombinant protein plasmid then transformed in bl21(de3) and used it for expression of my new mutant protein.I expressed it...

19 August 2016 8,032 6 View

What is the minimium possible time to reach OD 0.5 at 600nm by an E.Coli ?

08 August 2016 6,627 10 View

Is there any way I can improve AHL stock preparation for quorum quenching OPA based enzyme assay?

25 January 2016 2,213 3 View

Can someone suggest another scholar besides Hall, that defines hegemony as an ideological force that can produce "the othering"?

In this I mean other profound scholars that have defined hegemony as having the ability to represent those being dominated in a particular regime of representation.

28 January 2015 9,543 6 View

I am looking for scholarly literature on war journalists from developing countries. Can someone help and recommend some work ?

My readings for the past one year has revealed that war journalism is a predominantly western based. But have the developing world indulged in studying their journalists' who cover wars

06 January 2015 9,996 14 View

Can someone help me grasp the concept of memory in journalism?

Beyond Zelizer's work

05 January 2015 1,083 3 View

What is the colour of E coli colonies on EMB agar?

We got purple black, pink and white colonies on EMB agar plate. From that how we will detect E.coli.

10 July 2013 133 41 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View