I am interested in using a macrophage cell line for studies of ROS formation. Currently we are thinking of using RAW 264.7 cells or J774.2 cells. Are both cells lines real macrophages? Is there any difference between J774.2 and J774.1 cells?
What about THP-1 cells? THP-1 cells can be induced with PMA. I think there is good evidence for THP-1 cells induced by PMA to have a lot of macrophage features. But not sure about ROS in THP-1 cells.
Thanks Wensheng! We´ll look into these cells as well. They could be an alternative, although we would prefer to avoid PMA if possible. Anyway, many thanks!