PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

How to normalized a coutour plot in Abaqus?

I am trying to edit the odb file to get new data for the elements. I need to plot stress components divided over a specific value. Can it be done using python?

20 January 2021 9,557 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

Are chitosan nanoparticles biodegradable in the human body after parenteral administration?

There are numerous articles about development of various chitosan-based nanoparticles for parenteral drug/gene/vaccine delivery but I was unsuccessful to find any references about the...

17 December 2019 1,613 3 View

Why isn't blue-white screening used in gram-positive bacteria?

This question may be a little naive, but I've never seen anyone use blue white screening in a gram-positive bacteria before and wanted to know why! I know you need lacZM15 mutants in E. coli, but...

10 November 2019 7,084 2 View

Why is Enterococcus missing from 16S Illumina microbiome analysis?

Our research group is conducting 16S microbiome analysis with vaginal swabs, and we are interested in studying Enterococcus. For some reason, our swabs that are Enterococcus positive in culture...

10 November 2019 4,392 5 View

What are the possible ways to differentiate between VRE and Pediococcus?

I am using a media that is selective for vancomycin-resistant enterococci, but it keeps giving me Pediococcus (since Pediococcus is intrinsically resistant to vancomycin and has similar...

26 September 2019 403 4 View

Is it possible to get the list of bacteriophages against a given species of bacteria?

Hello, from this website (http://phage.org/names/2000/) it is possible to determine the genera targeted by a family of bacteriophages, for instance the phage species DS96 , H24 , M35 , P3 , P9 ,...

06 July 2019 6,924 7 View

Help me with DNA extraction from silicone oil ??

06 July 2019 2,581 3 View

What is the general upper detection limit CFU/ml for Enterococcus faecium?

How do you determine the upper detection limit for E. faecium? If there are too many to count on the plate, can I suggest that there are >25,000 CFU/ml for example? - or would that be too much

02 March 2019 1,261 1 View

How to check the genome sequence of locus A of Enterococcus feacium TX16 species;any site for this ?

website,genome sequence

01 February 2019 7,163 2 View

How to administer only single molecule of drug parenterally ?

I want to minimize possibility of anaphylactic reaction for hypersensitivity testing.

10 November 2018 8,477 2 View

What is the fate of large Mol. Wt. drugs that are administered in parenterals ?

Development of parenteral formulation of large Mol. Wt. (More than 800 Dalten substance.

10 November 2018 9,092 1 View