What is the best culture medium and growth condition (pH and temperature) for production of industrial bacteriocin by lactic acid bacteria including Enterococcus faecium, Lactobacillus salivarius, Lactobacillus plantarum, and Pediococcus acidilactici?
In my Ph.D. study, pediocin was produced from culture media (20% whey, pH=6 at 40C for 48 hours by the local isolate Pediococcus acidilactici.
I have previously used both MRS and BHI media to grow Lactobacillus plantarum and Streptococcus thermophilus at 37C.
Several studies have shown that whey supplementation enhances the production of bacteriocins significantly
Hello Dear as Alaa, MRS and BHI are the best media for bacteriocin production, specially when supplemented with( Glucose and other sugars). So, the optimum Temperature, Period and pH; 32-37℃, 16-24 hr, and 5.5-6.5
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