What is the best kit for preparing DNA of low copy number large plasmids (> 100kb) ?

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Pujic Petar

Macherey-Nagel NUCLEOBOND KIT . Use AX100 Catridges and use double volume of reactives for low copy number of plasmids. Usually , >~10 µg of plasmid DNA is amount obtained from 100 ml of E coli culture

Martin Schleef

Also the bacterial starting material is important. There is a huge difference between shaker flask cultivation and fermentation. If the plasmid is to be used for R&D, kit is ok. It is difficult to distinguish plasmid DNA from bacterial chromosomal DNA being co-purified in these systems as well.