I want to evaluate antioxidants levels of SOD, GSH, TAC, MDH and CAT. So I don't really know the best kits I should use considering the time, sensitivity and specificity.
https://dx.doi.org/10.1038%2Fnprot.2009.197
Article Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), ...
It might be helpful for you, Go through.
Thank you.
With Best Regards....!!!
We invented and evaluated the TAC (Total Antioxidant Capacity) - see https://omnignostica.at/product-details/dmp-20-4100-t-a-c
Antioxidants in the samples inhibit the attack of free radicals, initiated through a peroxide/peroxidase start-reaction associated with quenching of the colorimetric signal on the substrate tetramethylbenzidine. (Ref. doi:10.3390/antiox9010011)
I hope this might be helpful for you.
Respected Udo,
I have used Sigma Chemicals kits to determine these antooxidants and free radicals in vitro cell cultures and tissues of experimental mice. I very much appreciate Dr. Willibald, s original invention.
If you are interested in estimating these antioxidants and free radicals, please perform Digital Fluorescence Imaging employing Molecular Probes recently developed by Invitrogen or Becton-Dickinson. Good luck with your research.
Dean S. Sharma
Its better to optimize assays for that depending on the conditions
