How is it possible to get actual corrected numbers of Germans newly infected by COVID-19 due to the work of Matthias an der Heiden and Osamah Hamouda?

I prepare for each day a graph of the spreading of COVID-19 in Germany, the German state of Schleswig-Holstein, the region Kiel within Schleswig-Holstein and the administral ciecle of...

27 April 2020 6,032 3 View

How do I prevent cell loss during wash/fix/mount of rat lymphocytes?

I am culturing rat lymphocytes from whole rat blood, then washing in PBS followed by fixing in Carnoy's fixative. At this point I am mounting cells on a slide. Somewhere in this process I have...

20 January 2020 2,571 2 View

Plant gall nomenclature?

Hello, is there a nomenclature for plant galls (induced by parasites) which is generally accepted?

21 March 2018 1,509 3 View

Is this mycoplasma?

Dear Scientific Community :) A colleague recently tested his melanoma (ME1402) cells for mycoplasma and obtained some strange images of rod-like structures - quite different from the 'dot-like'...

14 December 2016 4,549 3 View

Can you TA clone an insert into pGEM T easy after designing primers with restriction sites?

Good day fellow researchers, I have recently designed primers with restriction sites which I am going to use to amplify my insert and clone into a reporter vector. After reading up on TA cloning I...

06 November 2016 3,733 10 View

Can anybody recommend a good antibody against Jun for WB and ChIP in rat?

Can anybody recommend a good antibody against Jun for WB and ChIP in rat? Thanks.

06 June 2016 4,247 2 View

Why do I obtain a strong Z-conrast in HAADF-STEM images?

What is the physical reason for the Z-contrast in HAADF-STEM images?

23 May 2016 4,675 3 View

Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

24 January 2016 4,301 3 View

Can you help me to understand molecular combing?

Hi colleagues! I need your help, because I have to understand the molecular combing method. I want to use the Molecular Combing system from Genomic Vision. Unfortunately, after reading several...

23 July 2015 4,229 5 View

Applications for Molecular Combing-Why not doing NGS or FISH?

Hi Colleagues, Molecular Combing enables the analysis of single DNA molecules on a glass slide. The DNA molecules are fixed on the glass slide and oriented like a line or fibre on the slide....

15 July 2015 4,151 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View