Working on viral infectious disease to evaluate different microRNAs profile using RT-PCR. I used mostly SV40 miRNA that's commonly used as an internal control, but sometimes in literature other internal controls used as U6, RNU44, U1, B-actin ,GAPDH etc.
Which type of internal control has the best accuracy for normalization instead of SV40?
Petra Disterer
A tiny bit of google Fu yields
https://www.thermofisher.com/uk/en/home/references/ambion-tech-support/microrna-studies/tech-notes/finding-the-best-endogenous-controls-for-real-time-quantitation-.html
It is a good idea to validate your control RNAs for every new assay - see
https://www.gene-quantification.de/miqe-index.html
for more information.
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