We are setting up a tumor model, injecting B16F10 into B6. Many people use simply PBS for the injection. I wonder if there is a preferred site of injection (flank, back, scruff, belly)? We are concerned that upon injection into the flank, the PBS solution does not stay put long enough (e.g. bleb seems to disperse fairly quickly).
The B16F10 melanoma sub-cell line (from the B16 model) is usually employed to set up "pseudometastatic models".
If you inject the B16F10 cells into the tail vein, you will have the development of marked lunh "pseudometastases" (see the attached article by Mathieu et al., 2007).
If you inject the B16F10 cells into the carotid, you will have the development of brain mets (see the second attached article).
For subcutaneous model, you should graft the cells "on" the flank and not "into", otherwise you will have an i.p. model that will rapidly "destroy" the mice.
You can also use the B16 model for s.c. grafting but it is necrosing a lot (see Darro et al., 2005, here attached).
You should contact my colleague Véronique Mathieu, MD, PhD at [email protected] (she is unfortunately not registered on RG ...). She can help you with "some important details".
Best regards
Robert
as above we have a SC model on the flank and an iv model into the tail vein for looking at metastasis. we have always used PBS for injections and that's been fine.
Have any of you successfully seen pulmonary metastases after subcutaneous injection of B16F10 cells? If so, how many cells would you recommend dosing? As someone mentioned earlier, I don't want the mouse to be euthanized due to tumor volume before lung metastases develop.
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