What is the best Hsp70 antibody to analyse Hsp70 in human colon carcinoma cells (LS174T)?

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Why there are multiple spikes around 50Hz (Power Interference) in frequency domain (fft)?

I am working on a project related to sEMG classification. In order to process the signal, I am trying to filter the signal, especially power interference. The signal is acquired from Delsys...

07 December 2020 9,745 10 View

What is the best way to plot Ct values for a gene expression in different Samples ?

Hi I want to plot a graph for Ct values from qPCR assay that resulted from a gene expression in different samples.

29 October 2020 1,384 3 View

How to filter resonance frequencies in Matlab?

Following Image presents the frequency domain of sEMG signal which was sampled at 4000Hz. However, unexpected noises were captured during the experiment. I believe that these are some sort of...

29 August 2020 7,421 3 View

What changers can occur if Arsenobetaine (Organoarsenic) digested with an acid (Nitric acid) ?

Arsenobetaine is the non toxin organic form of Arsenic commonly found in fish.If muscle of fish samples subject to a acid digestion, will non toxic nature be changed

30 May 2020 9,109 4 View

Any explanation about the effect of the pH value on SARS-CoV-2 Virus activity ?

Some studies reveal that SARS-CoV-2 virus activity can be mitigated at the alkaline pH medium (above pH= 7.5). Any explanation about the mechanism or the Chemistry behind this phenomenon.

28 May 2020 9,352 3 View

How to input a set of data from an Excel sheet into MATLAB Simulink?

I've got Solar irradiance data and Temperature data for a month in 1 hour time intervals in a Excel sheet. I've modelled a Simulink block set to get the Output of a PV array. Unfortunately, I'm...

27 May 2020 3,072 5 View

What is the best technique / extraction method for extract sesquiterpene coumarins from oligo-gum resins of Ferula asafoetida ?

Phyto-chemicals such as Conferone, Samarcadin, Bdrakemin Farnesiferol A, Fernesiferol C and Galbanic acid can be isolated from Ferula asafoetida. suggest proper extraction methods and solvent to...

22 May 2020 6,350 6 View

Can the sign of a coefficient change from + to - when treat for endogeneity?

Hi, I'm running a model to see the growth effects of the exchange rate. The coefficient that I got for the real effective exchange rate is positive significant under the fixed effect model and...

19 November 2019 6,677 1 View

How do I amplify a 7.4 KB fragment in mouse genomic DNA ?

I am struggling to amplify an 7.4 kB fragment which I need to confirm my CRISPR KO. I have tried Pfu and Phusion polymerase, used both HF and GC buffers, tried 50 - 70 C temp gradient, DMSO...

27 March 2019 9,699 3 View

How to design best primers to amplify a region in mice genomic DNA?

What is the database I should select under the Primer Pair specificity parameters in NCBI primer design tool?

14 March 2019 7,696 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Azmi Yerlikaya

We used Sigma-Aldrich Hsp70 antibody to determine the expression of Hsp70 in 4T1 mouse breast cancer line in response proteasome inhibition and got very good result. I am not sure whether the same antibody would work for colon cell lines. Good luck.

Saravana devi Sivanesan

Any polyclonal HSP 70 antibody with specific cross reactivity with human will work