What is the best endogenous control for qRT-PCR of microRNA from rat kidney samples?

More Pedro Paulo Gattai's questions See All

What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

How can technology help hospitalized children?

How do you think technology can help hospitalized children?

10 May 2024 6,508 2 View

Another opportunity to work in science area, anyone is interested?

One more opportunity of work on the science area. Title- Scientific Reviewer Duration – 12+months (extension) Location – Bethesda, MD Work Details: Complete the administrative review of grant...

08 May 2024 5,344 3 View

Is it appropriate to test presence/absence effects through a factorial design?

For factorial designs I usually see the independent variables being tested in levels (e.g., high/low). In my experiment, though, the effects of two different independent variables can only be...

05 May 2024 4,154 1 View

Is bitcoin the ultimate digital escape?

Topic from my first article published, download it to get full quality or check the publication page here: https://www.accept.pt/fundamental-concepts-of-global-society/

03 May 2024 6,414 0 View

Loss of Family Values: The Erosion of Society’s Foundation, what impact does it have on society?

Topic from my first article published, download it to get full quality or check the publication page here: https://www.accept.pt/fundamental-concepts-of-global-society/

03 May 2024 694 0 View

Educational System: The Pyramid of Workers, Not Thinkers, how does it affect the new generation?

Topic from my first article published, download it to get full quality or check the publication page here: https://www.accept.pt/fundamental-concepts-of-global-society/

03 May 2024 5,643 0 View

Why does inflation matter?

From my article: Inflation erodes the purchasing power of our hard-earned cash, leaving us with less value over time. Dollar, the most important currency, the reserve currency of the world,...

02 May 2024 3,326 3 View

What requirements (or qualities) are most important to be a good university professor?

22 April 2024 3,009 5 View

Vacancy of manager of bioinformatics in USA, are you interested?

Dear ResearchGate community, I received the contact of a recruiter in my email regarding another vacancy in USA. Title: Bioinformatics Training Program Manager Duration: 12 months Location:...

18 April 2024 1,544 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Ethylene glycol is newtonian or non newtonian fluid?

Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.

09 August 2024 2,111 2 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View