What is the best endogenous control for differentiating PC12 cells?

05 May 2016 1 5K Report

I am working with PC12 cells and I take mRNA samples every 48 hours after differentiation with NGF. Which would be the best endogenous control for qPCR given that it needs to be one that does not change upon NGF treatment? Thanks!

Andre Luiz Zorzetto-Fernandes

Here are some exemples for PC12 but I recommend you order the primers for at least 3 of them and check their expression under your conditions.

http://link.springer.com/article/10.2478%2Fs11532-012-0164-5

http://www.ncbi.nlm.nih.gov/pubmed/20113474

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