We would like to electroporate neurons in organotypic slice cultures with DNA for genetically-encoded flourescent reporters (pH reporters, GEVIs etc). We would like to use an anionic dye that can help us visualize the electroporation but won't hang around in the cell so long that it would interfere with the later visualisation of the expression of the genetically-encoded flourescent reporter several days later. We worry that if we use something like a Alexa-flour 488 Dextran this will hang around so long that it would interefer with later flouresence measurements from the genetically encoded reporters. Any suggestions would be greatly appreciated.
We do a lot of single-cell electroporation and routinely include one of the non-Dextran Alexa dyes - 488 or 564 usually. Never seemed to have any problem with the fluorophore. Probably the Dextran dye also won't cause any problems but I never tried it...
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