I have been attempting to extract DNA for barcoding on various macrobenthos and have at present been unsuccessful. I can only make use of one/two legs per specimen e.g of an amphipod. I am using the CO1 gene. I have tried the Qiagen blood and tissue kit, chelex method and the zymo research tissue and insect DNA microprep.
Are there any other kits/methods which would be useful to try?