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Questions related from Matthew Frias
Hello, I've been having issues with my standard curve and wonder what the possible issue with it could be. plasmid standard with starting concentration determined to be 2E8 copies/ul previous...
02 February 2020 2,730 2 View
I have a plasmid in which I want to calculate the molar mass per base pair ( (g/mol)/bp ). I also have the %GC and the plasmid length (in bp). How do I calculate the (g/mol)/bp?
10 October 2018 4,120 0 View
I ran a full plate of qPCR standard yesterday and had no amplification. I was running a gradient on the plate to test the different annealing temperatures. When the ran finished it showed no...
06 June 2018 795 5 View
Hello, My colleague and i have a linearized double stranded DNA fragment that we want to amplify through ddPCR. The problem is we're only getting a %50 recovery with it. We've double checked and...
06 June 2018 6,655 4 View