I already used the MS medium culture, and I had an induction of callus, and I want to try another culture medium if possible.
MS is a good start, but culture media depends a lot on the species you're working on. I would advise to look first if there are any publications on the species you are working on and see what they use. Many culture media are variations of MS, with changes in nutrient concentrations or in growth regulators.
I know of works done with coconut water culture media instead of MS, but this could be tricky, as there is variation because not all coconuts are created equal.
I would check what is available in the literature for the species you are working, or if unavailable, in some other related species.
Good luck with your quest!
I work on forest species (tree), I read some article on in vitro cultures of trees, and generally the most used medium: the MS and MS / 2 medium, and as you said, with a change in concentration of growth hormone
I'd like to use another culture medium to see the response of embryos, and compare the results
Hi, Massakib,
MS medium is quite good for induction of non-organized growth (callus), but for may prevent plant organogenesis. Please, remember, that MS has been developed for tobacco callus and far from optimum for other purpose.
You have to reduce nitrogen, and significantly reduce chloride, whcih is micro nutrients only.
B5 medium is much better, but you have to replace CaCl2 on CaNO3.
Good luck!
.
I would recommend the use of B5 medium (Gamborg); but not at the higher ionic strength (complete salts). In our laboratory we use B5 medium to half its ionic strength (B5 / 2) to germinate seeds of Capsicum chinense and there is a good growth of the roots of this plant. We have also used the MS and B5 medium at different ionic strength for culturing zygotic embryos of Coffea arabica (Coffee); even we added different concentrations of growth regulators and callus induction and different morphologies observed in embryos.
Keep in mind that by decreasing the ionic strength of the medium is necessary to use agar, decreasing the polymerization medium capacity if another gelling agent used as gelrite.
Another important point is the concentration of the carbon source; we usually use concentrations from 3% to 0.5% sucrose; because they can present problems vitrification.
I agree with Jesus, B5 medium is much better to compare with MS. For the vitrification problem, you can try to use better ionic balance and air excange system. You can use cotton top for bettw+er aeration.
I also agree with jesus and Taras, Gamborg's B5 medium is much effective than MS. especially in embryo culture techniques.
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