How can I keep fresh plant material for enzyme and phytohormone assays and gene extraction?

Greetings, I have to transport fresh vegetable matter for enzymes and phytohormones assay and gene extraction in a laboratory far from my laboratory, so how to keep this vegetable matter in its...

09 October 2019 10,044 5 View

What are the extraction and dosing techniques for bacterial osmoprotectants?

Greetings, I'm searching for methods to extract and dosing some of bacterial osmoprotectant such as ectoin and betaine glycine and proline from bacteria Thank you

11 December 2018 7,824 0 View

How find the same Osmotic pressure of two solutions of NaCl and PEG 6000 ?

I need to find the same osmotic effect (pressure) on a plant or seed exerted by two solutions one contained Nacl and other PEG 6000 ! how can i find the equivalence between NaCl and PEG 6000...

02 March 2018 7,345 7 View

How can i disinfect seeds without being imbibed or stressed by disinfection solution?

How can i disinfect seeds use to germination study under stress conditions (water stress or salinity) without being imbibed or stressed by disinfection solution? Thank you

02 March 2018 3,027 2 View

Does the disinfection of the soil make him lose these characteristics?

I need to work with disinfected soil, does putting this soil in an autoclave make it lose these characteristics? Is there any other techniques to obtain disinfected soil, without any microorganism?

31 December 2017 4,893 15 View

What's the best software to compare?

31 December 2015 5,650 0 View

Why don't we use colchicine to kill directly tumors instead of starving these cells?

11 December 2014 8,687 11 View

I'm looking for a protocol to stain and view chromosomes in metaphase?

I'm looking for a protocol for viewing - under a microscope - the chromosomes in metaphase stage, in a plant root after seed germination.

05 June 2014 2,039 8 View

Can we regenerate human organs?

Plants and some animals are characterized by the system of regeneration and repair of damage in their bodies. Is it possible to use this feature for regeneration of human organs and regenerated...

04 May 2014 4,324 10 View

What is the best culture medium used in the in vitro culture of zygotic embryos of plants?

I already used the MS medium culture, and I had an induction of callus, and I want to try another culture medium if possible.

03 April 2014 7,537 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View