I m doing a studies about the effects of PGPR on seeds and plants developing, by inoculation, so what's the best way to inoculates seeds by bacteria!
Thank you
Greetings, I have to transport fresh vegetable matter for enzymes and phytohormones assay and gene extraction in a laboratory far from my laboratory, so how to keep this vegetable matter in its...
09 October 2019 10,167 5 View
Greetings, I'm searching for methods to extract and dosing some of bacterial osmoprotectant such as ectoin and betaine glycine and proline from bacteria Thank you
11 December 2018 7,945 0 View
I need to find the same osmotic effect (pressure) on a plant or seed exerted by two solutions one contained Nacl and other PEG 6000 ! how can i find the equivalence between NaCl and PEG 6000...
02 March 2018 7,469 7 View
How can i disinfect seeds use to germination study under stress conditions (water stress or salinity) without being imbibed or stressed by disinfection solution? Thank you
02 March 2018 3,170 2 View
I need to work with disinfected soil, does putting this soil in an autoclave make it lose these characteristics? Is there any other techniques to obtain disinfected soil, without any microorganism?
31 December 2017 5,021 15 View
what is the best software for comparison between two things : images, structures, database ... Thank you
31 December 2015 5,764 0 View
As the researchers could change the molecule colchicine to make it inactive in the body until it reaches the tumor and becomes active to break the blood vessels supplying the tumor, and this...
11 December 2014 8,788 11 View
I'm looking for a protocol for viewing - under a microscope - the chromosomes in metaphase stage, in a plant root after seed germination.
05 June 2014 2,149 8 View
Plants and some animals are characterized by the system of regeneration and repair of damage in their bodies. Is it possible to use this feature for regeneration of human organs and regenerated...
04 May 2014 4,454 10 View
I already used the MS medium culture, and I had an induction of callus, and I want to try another culture medium if possible.
03 April 2014 7,654 7 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View