What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

22 January 2021 1 3K Report

Hi everyone

I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and incubation time for coating the dishes with Pam3.

Thanks

Malcolm Nobre

Hello Akram

Why don't you do a few pilot experiments if you have the resources with the following concentration and time of incubation.

Concentration of Pam3CSK4 :

Concentration range from 200ng/ml to 1000ng/ml.

Try three time points :

30mins, 1hour and 2 hours.

All the best.

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