For a standard BCA assay, I am checking if the protein detection can be fine-tuned without adding any other material as found in other protocols.
In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?
13 July 2024 6,296 5 View
While deriving for a momentum equation with elastic stress effects, I am planning to put a ratio between elastic modulus (in Pa) and dynamic pressure (in Pa) in a simplified form. I am not...
23 June 2024 8,059 1 View
I have scan data of multiple individuals over a while and their positional photographs were taken at certain fixed instances during focal sampling. I want to obtain the inter-individual distances...
26 May 2024 2,597 2 View
I want to know if there any proper method available for choosing of non-conserved relaxation parameters in MRT-LBM? Any literature suggestion would be helpful in that case.
18 April 2024 939 0 View
Hi! My thesis is primarily quantitative in nature. I would be using pre-validated scales for measuring my variableness. I would like to add a qualitative component to it. My query is if there is a...
30 May 2023 7,894 4 View
Let us consider a case where we need to simulate a moving cylinder in a microchannel where the Reynolds number is order of 10^(-3). Is the Entropic LBM useful in that case?
28 May 2023 3,712 0 View
For calculating the stress tensor for a non-Newtonian fluid, the factor D_{alpha}{beta} is written in terms of distribution functions. Why does one need to calculate using non-equilibrium...
17 April 2023 8,551 0 View
Hello Everyone! I am optimizing the ATAC-seq protocol currently with ProB cell lines following the Kaestner Lab protocol which is a modified version of the Buenrostro et al. protocol. I have...
03 May 2022 2,906 0 View
While calculating mediation by Hayes process macro on SPSS, we obtain the outcomes with coefficients, LLCI and ULCI, etc.. Which value should we take for commenting on the degree of mediation?...
10 January 2022 4,333 1 View
I am working with a herbal extract and currently attempting to determine the appropriate concentration to use for further work, provided it does not cause cell-death- using cellular viability for...
16 December 2021 4,610 7 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View