Sayantika Sanyal

2 Questions 3 Answers 0 Followers

Questions related from Sayantika Sanyal

How can I standardize protein purification range for small-scale purification?

I am working on extracting proteins from E. coli cells. However, the native proteins are present in very small quantities, making extraction challenging and increased risk of yield loss. To...

30 July 2025 5,707 3 View

What is the best concentration for BCA reagent preparation for protein estimation and how can be fine tuned for low concentration of proteins?

For a standard BCA assay, I am checking if the protein detection can be fine-tuned without adding any other material as found in other protocols.

24 September 2024 427 0 View