How to derive parametric equations from a given dataset by Gene Programming Method ?

I have a dataset of some dependent and independent variables. How to get a parametric equation from this. In some paper, it is written that they have created equation from Gene programming method....

24 July 2020 6,979 2 View

How to decide the proper number of elements in edge sizing for tetrahedral meshing ?

For hexahedral meshing, it is quite simple to divide one edge in a particular number of division. But for tetrahedral meshing it is not that simple. If we are using a complicated domain for...

29 June 2020 4,137 6 View

Is it possible to create an analytical expression from the artificial neural network toolbox in MATLAB?

Is it possible to create an analytical expression from an artificial neural network toolbox in MATLAB? If not, then how can these datasets be used as objective function in optimization? In some...

10 June 2020 5,753 8 View

How to plot the graph of turbulence intensity in ansys cfx for steady state analysis??

I want to plot the graph of turbulence intensity vs height of domain. How to do that in Ansys cfx for steady state analysis?

20 April 2020 1,993 3 View

What will be the analytical solution for two-dimensional unsteady-state heat conduction equation for a cylindrical pipe/rod (cylindrical coordinates)?

Is there any article that provides good insight on the procedure for reaching an analytical solution of this problem, especially when heat is applied at the wall of the cylindrical pipe and the...

04 March 2020 9,092 7 View

Can someone please suggest a circuit to prevent back powering in jtag connections when working with FPGA devices?

I need some clarity on this topic as to how we can implement jtag protection while programming/debugging FPGA circuit.

28 February 2019 6,070 3 View

Drag or Lift Coeffcient Calculation in ANSYS FLUENT?

What is the value of velocity which ANSYS takes up while it calculates drag or lift coefficients when a fully developed flow, involving power law fluid, enters inside a parallel plate. Is it the...

07 February 2019 162 2 View

How can I change outflow boundary condition to convection boundary condition in ANSYS FLUENT ?

I am trying to implement CBC in ANSYS Fluent at the outlet. Please guide me through. I have written a single UDF where I have defined a parabolic profile at inlet and a CBC for the outlet. When I...

29 December 2017 4,835 2 View

ER stress induction in Hap1 cells?

26 April 2016 7,145 3 View

Does anyone have experience with transfection gRNA and cas9 mRNA using RNAimax?

Does anyone have experience with transfection gRNA and cas9 mRNA using RNAimax? What would I select the transfection positive cells with, given that I'm transfecting RNA?

25 February 2016 1,865 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View