What is the best collagen coating protocol that uses acetic acid?

11 November 2017 0 381 Report

Specifically: What is the optimum concentration of rat tail collagen I? How long should the collagen solution be left on the plate? Should I wash the plate with PBS? How long should drying take place? Should I partially remove the lid to dry and/or use UV to dry the plate? Do plates need to be stored at 4 degrees C?

Protocols vary on this subject. Which protocol has worked best for you?

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