What is the best cell-line other than HEK-293 for delivering vector containing guide?

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Choosing an appropriate TLD for mixed radiation?

Could you please recommend the appropriate TLD card model for both personal and environmental dosimetry in a high-energy proton accelerator center, specifically for dosimetry of beta, gamma, and...

17 June 2024 1,958 0 View

Is Rough Set Theory or Neutrosophic Logic better for machine Learning healthcare models handling discrete data and needing simplicity?

In the context of machine learning models for healthcare that predominantly handle discrete data and require high interpretability and simplicity, which approach offers more advantages: Rough...

21 April 2024 9,505 2 View

How can the integration of Rough Set, Neutrosophic, and Fuzzy theories enhance decision-making processes in reverse logistics supplier selection?

In my thesis on reverse logistics, I explore the use of the ANP-TOPSIS hybrid method in handling uncertainty. I'm interested in hearing from researchers about the effectiveness of 'Rough Set,'...

20 April 2024 1,590 1 View

What are the best phase change materials with the highest performance for use in asphalt to reduce the heat island effect?

Phase change materials should be economical and affordable

23 March 2024 3,407 2 View

Could anyone help me with HYSYS software?

I wanted to simulate article titled "Simulation of multi-method CO2 capture and purification process" using Aspen HYSYS software. However, I encountered a lack of data. If possible, could anyone...

18 January 2024 7,981 0 View

Does anybody know a Model-Based System Engineering real case study in Automotive Driving?

I need to re-implement a case study in automotive systems (recommended), on "APP4MC" platform, with a reasonable level of complexity in hardware and software characteristics. If anybody knows an...

24 November 2023 7,941 2 View

In rna extraction with trizol why the upper phase is red, phenol-chloroform phase and the lower phase is aqueous ?

21 November 2023 3,305 5 View

Quality assurance protocol for clinical laboratory between instruments results comparison?

Our hospital lab uses 5 biochemical auto analyser for patients samples analysis. Which quantity assurance protocol is best for the agreement of results of these instruments? Please examlify with...

20 October 2023 4,574 1 View

What is the best protocol of RNA( lncRNA) extraction using trizol reagent from plasma ?

I used this protocol but I did not answer 1) add 250 µl plasma to 750 µl Triazole 2) 5 min incubation 3) add 150 µl chloroform, mix 12 sec, 3 min incubation 4) Centrifuge at 10,000g for 10 min at...

11 August 2023 4,191 0 View

What's your idea and suggestion for my recombinant protein expression and purification?

Hello everyone, I'm currently trying to overexpress and purify a human hormone that is a member of the TGF beta superfamily (536 a.a without first 24 a.a signal peptide) with 7 cysteine amino...

21 June 2023 1,557 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View