Farshid Amiri

3 Questions 6 Answers 0 Followers

Questions related from Farshid Amiri

What is the best solution for pH adjustment of HEPES A?

I am going to make a HEPES A lysis buffer (50 mM HEPES, 500 μM ZnCl2) but as you know, ZnCl2 is an acidic solution and I need to increase the pH from 5 to 7.0. When I want to adjust it with NaOH,...

13 June 2019 3,344 8 View

Does anyone have experience with hematopoietic stem cell culture?

What are the best culturing media, supplements, and protocols for expansion of HSCs?

17 April 2018 8,995 8 View

What is the best cell-line other than HEK-293 for delivering vector containing guide?

Has anyone tried to deliver vector to cells other than HEK-293? What was the efficiency of transfection?

13 November 2016 835 2 View