What is the best cell-line model for neurons?

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A paper on a fossil lycopod?

Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...

07 August 2024 4,857 2 View

Dear colleagues, Can anybody by chance have pdfs of two books in Chinese: Liu (2003) and Zhang et al. (2004)?

Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression，Tarim Basin of Xinjiang，NW China. Palaeontologia Sinica， New Series A，no. 14（Whole no. 190）....

28 July 2024 6,390 1 View

How to apply Kogut-Singh Index to measure cultural distance in intercultural dyads?

In my current study I'm measuring cultural diversity in intercultural coaching dyads by calculating cultural distance (Kogut-Singh Index, 1988) between coach and coachee in each intercultural...

03 June 2024 9,990 1 View

Как найти свой Researcher ID?

30 May 2024 6,369 0 View

How can I prepare electrospun webs for BET analysis?

How should I prepare electrospun fibers for BET analysis? How much (cm x cm) should I cut to obtain reliable results? What about degassing? I have PLA sample, 40 C will be enough? Thank you

23 April 2024 7,364 2 View

A pdf of Coquel & Brousmiche Delcambre (1996)?

Dear colleagues, Does anybody by chance have a pdf of this paper? Coquel, R., & Brousmiche Delcambre, C. (1996). Comparaisons spores in situ-spores dispersées chez quelques Equisétales,...

19 March 2024 8,258 3 View

How to represent qPCR data when a gene is not expressed in a group?

In my experiment, one group didn't have Cq when a use a specific primers to specific gene. But another groups was ok. How can i represent this results on the graph? I need to put Cq = 0 or Cq = 40?

17 March 2024 6,789 0 View

CD markers expression patterns?

Hi everyone, could you recommend any article on CD marker profile changes and their biological significance due to this change? I have been looking for something but can't find anything. In my...

10 March 2024 3,702 0 View

I can't see my purified PCR product in an agarose gel?

I successfully amplified fungal ITS from soil samples, however after running the purified PCR products in an agarose gel they are barely visible and don't look like defined bands but rather...

10 March 2024 8,377 2 View

LFP | Graphite pouch cell. Why is not discharging?

I'm stating to assembly LFP

14 January 2024 7,199 5 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Awanish Kumar

Claiming for the best cell-line model is very difficult and in my opinion it depends very much on the aims and objectives of the experiment that you are going to perform. Some neuronal cell lines are PC12, SH-SY5Y, Neuro2a cell line.

Natalia Murataeva

Awanish Kumar do you think Neuro2a would work well for suspended cell assays?