Need suggestion for stable cell line generation?

Hello All! I am trying to generate HCT 116 stable cell line. I studied many protocols and proceeded as follow: 1) I cultured cells in 6 wells plates at density of 5 x 10^5. 2) At 70% confluency,...

10 November 2016 9,838 4 View

PRPF4B (NM_003913) Human cDNA Clone?

05 June 2016 9,725 1 View

How to efficiently collect macrophages from mouse blood?

I am trying to collect macrophages from the mouse blood, but till now I am unable to do this perfectly. I feel following problems and need some recommendations: 1) Blood in mouse is very less,...

10 November 2015 9,538 2 View

How can I get the data from this western blot picture?

Hello everyone, As you can see in the picture, I have run two samples on SDS-Page, and signals have been detected by PARP antibody. Control has no band, while the treated sample is giving band,...

05 June 2015 9,134 8 View

Has anyone tried stable transfection of HCT-15 cells?

I am in search of recommendations about stable transfection of HCT-15 cells regarding selection of vector and antibiotic. It's my first time with this experiment and and I am searching a suitable...

05 June 2015 2,872 2 View

Why am I always getting dark background in western blot?

04 May 2015 5,864 9 View

How can one isolate cell membrane proteins from mammalian cells?

I would like to isolate cell membrane proteins from human colorectal cancer cells (HCT-15) and human leukemia cells (HL-60). I am in search of a suitable protocol. I have to order all the...

02 March 2015 7,606 8 View

Is Decursin Angelate available commercailly?

Hello All, I want to work with Decursin angelate, but i am not able to find it commercially by some company. Can any one tell me about its commercial availability?

02 March 2015 8,634 0 View

Does anyone have sample for Digital Data of Cell Cycle Histogram?

We know that flow cytometer giving us a histogram of cell cycle, uses some data from individual cells. Often these are FCS files. I mean that flow cytometer records the fluorescence of individual...

01 February 2015 7,555 2 View

Did someone use "Prostaglandin E2 Express EIA KIT"?

01 February 2015 8,162 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

No title

02 March 2021 3,060 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View