I am currently working in the process of automating our research lab to be able to process a higher number of samples. Our work consists in detecting and quantifying pathogens in mammalian samples (e.g., blood, faeces, tissue, swabs). As such, I am interested in an automated extraction system which produces good DNA/RNA yields to be sent for NGS.
Until now, we have been using Qiagen kits for our manual extractions, so I thought that a machine from the same brand would do for us. However, I've been told that the QIAcube Connect does not really take that much work out of your hands, and that the sample volume obtained at the end of the process with the QIAcube HT is way lower than the one with the manual kit. I have also checked other machines, such as Thermo Scientific's Kingfisher Flex and its kits, but do not know how well they do in comparison with Qiagen's kits.
Based on your experience, which automated extraction system would you recommend? And which brand of kits have you used with it? The system and kits do not need to be from the brands mentioned here (as long as the produce good results).
Thank you very much in advance.
Hi Laia-M. Pardinilla ,
From my experience, I prefer instruments based on magnetic beads technology to those based on silica columns (column clogging problems particularly with blood samples). In addition, I will opt for an open system where I can use reagents according to my choices/needs and upload my own extraction programs. Good luck
Check out this system, I haven't tried it but it may interest you:
https://www.purigenbio.com/ and https://www.purigenbio.com/applications/ionic-ffpe-pure-dna
Dear Laia-M. Pardinilla , I am agreeing with the answer of Aissa Saidi for adopting open system where you can modify the program accordingly. In my opinion, the Qaigen provides multiple options for you to choose one based on your output of sample processing.
Laia-M. Pardinilla back when i worked in the diagnostic laboratory, i used CyBio Felix from AnalyticJena. It is also based on magnetic beads technology and it is fully automated, you can modify the program, customize configurations.. i really liked that one https://www.analytik-jena.com/products/liquid-handling-automation/liquid-handling/flexible-benchtop-liquid-handling/cybio-felix-series/
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