Laia-M. Pardinilla

6 Questions 4 Answers 0 Followers

Questions related from Laia-M. Pardinilla

Does the presence of DNA affect the RNA-to-cDNA conversion?

We are currently working on a project involving the extraction of DNA and RNA from various types of animal samples, such as whole blood, serum, faeces, etc. The objective is to detect various...

18 December 2023 5,326 5 View

What is the best automated nucleic acid extraction system?

I am currently working in the process of automating our research lab to be able to process a higher number of samples. Our work consists in detecting and quantifying pathogens in mammalian samples...

10 May 2023 9,870 4 View

How many samples can be pooled together for NGS?

We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...

17 February 2021 5,740 4 View

Is there any alternative to a fume hood when working with DTT?

I am going to work with dithiothreitol (DTT) whilst isolating DNA and RNA, so I was looking for a safe alternative to a fume hood, such as maybe a specific type of mask or anything different that...

18 February 2020 3,846 3 View

Faecal sample storage: which one is better, RNAlater or ethanol 95%?

I want to isolate DNA and RNA from feline faecal samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. The isolation will not be...

23 September 2019 3,171 4 View

Are HEPA filters essential for DNA and RNA isolation?

I want to isolate DNA and RNA from faecal and blood samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. I currently have one PCR...

22 September 2019 7,852 4 View