Hello everybody
My research group and I are trying to do an immunofluorescence for NOGO-A protein, but we are not getting the markings right.
For the technique, we used antigen retrieval with citrate buffer at a 25% tween concentration, which we assume is too concentrated.
If you have an efficient antigen retrieval protocol for NOGO-A labeling I would be grateful.
Yours sincerely,
Ana Beatriz