02 July 2019 3 6K Report

Hello, I am looking for a suitable antibody for hGH. I wanna to confirm my result with a confirmatory test such as Western Blot.

Thank you.

More Zahra Damirchi's questions See All
How to completely dissolve soy lecithin?

Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...

02 August 2024 6,980 4 View

When using NVivo software for data analysis, is it necessary for the researcher to also manually analyze the data?

it's my first time using NVivo software.

01 August 2024 9,304 5 View

How to convert a potential collected with reference to Saturated calomel electrode to Reversible Hydrogen Electrode?

I have collect a LSV data by taking SCE as the reference electrode. But I want to convert this one to Potential with respect to Reversible Hydrogen Electrode. Please suggest me the required...

13 July 2024 7,841 2 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

Is it a source for moisture factor in different plants?

Is it a source for moisture factor in different plants? I need to find moisture factor (oven dry weight / wet weight) in plants in different studies.

10 June 2024 842 0 View

How can I clean autoclave (PTFE) from sulfur contamination ? in all aspect of color, smell and existence of Nano particle of sulfur??

the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...

31 May 2024 2,985 0 View

What's the optimal temperature for freezing a lipopeptide solution before lyophilization?

I have a lipopeptide solution that I intend to lyophilize in a few days. Should I put it in the -20 freezer or -80? Which temperature is best for maintaining its properties?

20 May 2024 3,893 0 View

Challenges in Identifying Crack Initiation and Interpreting Results in Abaqus Direct Cyclic Fatigue Simulation?

Dear researchers, I'm currently conducting a direct cyclic fatigue simulation in Abaqus, but I'm encountering difficulties in analyzing the results. Despite the simulation running, I'm struggling...

04 May 2024 6,957 2 View

Cloudy background of polyacrylamide gel after de staining, a confusing problem?

Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...

02 May 2024 895 2 View

Fail OCR measurement for my ReN differentiated cells?

I seeded 30000 ReN progenitor cell on XF96 plate which coated with matrigel. I differentiate them for 2 weeks and read OCR with inhibitors. However nothing worked. FCCP and oligomycin were same...

17 April 2024 9,012 0 View

Similar questions and discussions
Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close